List of Gateway vectors for the BiFC assay
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Enhanced cyan fluorecent protein (ECFP), EGFP, EYFP and monomeric RFP1 (mRFP1) can be divided into two fragments. The letters ‘n’ and ‘c’ represent N- or C-terminal fragments of a split fluorescent protein, and the letters ‘C’, ‘G’, ‘Y’ and ‘R’ represent the type of fluorescent protein (ECFP, EGFP, EYFP or mRFP1). Since the nucleotide sequences of C-terminal CFP and C-terminal GFP are identical, the fragment is designated as cCG. The N- and C-terminal fragment from mRFP1 contain myc- and hemagglutinin-epitope tags, respectively. |
pUGW-based BiFC vectors |
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pB4 and pB5 series |
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References
Kamigaki, A., Nito, K., Hikino, K., Goto-Yamada, S., Nishimura, M., Nakagawa, T., and Mano, S. (2016). Gateway vectors for simultaneous detection of multiple protein−protein interactions in plant cells using bimolecular fluorescence complementation. PLOS ONE 11, e0160717. [open access]
Tanaka, Y., Kimura, T., Hikino, K., Goto, S., Nishimura, M., Mano, S., and Nakagawa, T. (2012). Gateway vectors for plant genetic engineering: overview of plant vectors, application for bimolecular fluorescence complementation (BiFC) and multigene construction. In Genetic Engineering – Basics, New Applications and Responsibilities – Edited by Hugo A. Barrera-Saldaña, InTech, pp.35-58. [open access]